LifeCanvas Introduces DALISPIM: Pioneering Open-Top Light Sheet Microscope for High-Throughput 3D Imaging
LifeCanvas Technologies has once again pushed the boundaries of spatial biology and 3D imaging with the launch of DALISPIM, an innovative open-top light sheet microscope. Designed to bring unprecedented versatility, speed, and precision to biological imaging, DALISPIM is poised to redefine high-throughput workflows for drug discovery, disease modeling, and tissue analysis.
At the heart of DALISPIM lies LifeCanvas’s patent-pending Depth Adaptive Light Interface (DALI) technology—a groundbreaking approach that eliminates traditional imaging constraints by enabling seamless, aberration-free imaging directly within standard sample carriers. From multiwell plates to organ-on-a-chip devices, DALISPIM transforms how scientists capture and analyze complex biological structures in three dimensions.
Redefining Accessibility in 3D Imaging
LifeCanvas developed DALISPIM with a clear mission: to make high-resolution volumetric imaging more accessible, intuitive, and integrated with modern experimental workflows. Traditionally, light sheet microscopy has been limited by sample preparation complexity and the need for immersion-based imaging environments. DALISPIM changes this paradigm by introducing an open-top architecture that simplifies the imaging process without compromising image quality or speed.
This unique design allows researchers to directly image tissues and samples in their existing containers—such as well plates, Petri dishes, and slide glasses—reducing sample handling time, preserving biological integrity, and supporting high-throughput studies. By merging the familiarity of an inverted microscope platform with the advanced capabilities of light sheet imaging, DALISPIM bridges the gap between convenience and performance.
Powered by DALI: A New Optical Frontier
The core innovation of DALISPIM, the Depth Adaptive Light Interface (DALI), represents a significant leap forward in imaging technology. DALI dynamically adjusts to the optical properties and depths of biological samples, ensuring consistently sharp and distortion-free images across varying tissue thicknesses. This means scientists can visualize structures up to 6–8 millimeters deep without the need for immersion baths or refractive index matching solutions—steps that often complicate traditional imaging workflows.
With DALI, DALISPIM delivers aberration-free volumetric imaging across a wide range of biological specimens, including intact organs, organoids, tissue sections, biopsies, and model organisms. This versatility empowers researchers to perform imaging directly in multi-sample configurations, such as multiwell plates or organ-on-chip devices, facilitating the exploration of multiple biological conditions in parallel.
Speed and Precision Without Compromise
In addition to its optical innovations, DALISPIM integrates patented axial sweeping technology (Dean et al., 2015) to enable rapid, high-resolution imaging over large volumes. This technique dynamically shifts the light sheet through the sample, maintaining uniform illumination and sharpness throughout the imaging field. The result is consistent, high-quality image data at remarkable speeds—ideal for applications demanding throughput, such as drug screening or large-scale tissue mapping.
Whether capturing detailed structures in neuronal networks, vascular systems, or organoid models, DALISPIM combines speed, precision, and scalability, ensuring researchers can image more samples in less time without compromising data fidelity.
Simplifying Imaging from Start to Finish
LifeCanvas understands that even the most powerful imaging systems must be accessible and user-friendly. DALISPIM’s intuitive software interface streamlines every step of the imaging process—from system alignment to acquisition and data reconstruction. Step-by-step setup instructions and guided workflows make it easy to define imaging boundaries, set acquisition parameters, and execute complex imaging sequences with minimal training.
The software also includes advanced tiling and stitching capabilities, allowing users to seamlessly navigate across large tissue areas and reconstruct entire organs or organoids in three dimensions. Researchers can visualize, analyze, and export volumetric datasets using LifeCanvas’s integrated post-processing suite, reducing reliance on third-party software and ensuring consistent data quality.
A Complete End-to-End Imaging Ecosystem
DALISPIM is not just a microscope—it’s part of a complete imaging solution designed by LifeCanvas to support researchers from sample preparation to data analysis. The system integrates seamlessly with LifeCanvas’s sample processing pipeline, which includes advanced tissue clearing, staining, and labeling reagents optimized for light sheet microscopy.
For large-scale or collaborative projects, LifeCanvas offers MOSAIC, a high-performance, networked data storage solution built specifically for managing and analyzing massive light sheet datasets. Together, DALISPIM and MOSAIC form a comprehensive ecosystem that enables smooth transitions from image acquisition to visualization, analysis, and data sharing—accelerating research timelines and enhancing reproducibility.
Unlocking New Possibilities in Spatial Biology
The introduction of DALISPIM arrives at a critical time for the field of spatial biology, where understanding the spatial context of cells, tissues, and organs is essential for breakthroughs in areas like neuroscience, oncology, immunology, and regenerative medicine. As researchers increasingly seek to explore biological systems in their native 3D environments, DALISPIM provides the tools needed to uncover cellular architecture, signaling pathways, and disease mechanisms at unprecedented resolution and scale.
By combining open-top design, adaptive optics, and automated acquisition, DALISPIM empowers scientists to conduct experiments that were previously constrained by the limitations of traditional imaging technologies. Its compatibility with standard laboratory carriers also facilitates integration into existing workflows, minimizing disruption while maximizing discovery potential.
Built for Innovation and Collaboration
True to LifeCanvas’s mission, DALISPIM embodies the company’s commitment to democratizing advanced imaging technologies. It provides a scalable, accessible solution that serves a diverse community of researchers—from academic labs exploring fundamental biology to pharmaceutical companies developing new therapeutics.
With expert support, comprehensive training, and a growing community of users, LifeCanvas ensures that every DALISPIM system delivers consistent, high-quality imaging performance and long-term reliability. Researchers can also consult directly with LifeCanvas imaging specialists to optimize system configuration, adapt workflows for unique sample types, and extract meaningful biological insights from volumetric data.
The Future of 3D Bioimaging Starts Here
The launch of DALISPIM marks a significant milestone in LifeCanvas’s ongoing mission to advance imaging, accelerate discovery, and transform understanding of biological complexity. By making high-throughput 3D imaging more intuitive, accessible, and accurate, DALISPIM sets a new standard for how researchers visualize and analyze life at the cellular and tissue level.
